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Oral squamous cell carcinoma microarray analysis elucidates gene expression(Science Letter Via Thomson Dialog NewsEdge) Gene expression alterations in oral squamous cell carcinoma (OSCC) have been identified through microarray analysis. "Oral squamous cell carcinoma (OSCC) is a common type of human cancer, but little is known about the molecular mechanisms" underlying this malignancy, pathologists in Japan explained. "Comprehensive gene expression profiling is essential for understanding OSCC," argued H. Tomioka and colleagues at Tokyo Medical & Dental University. In their study, "cDNA microarray was used to analyze expression patterns of 16,617 genes in nine OSCC patients." "Forty-seven genes with altered expression among all cases were extracted," the scientists wrote in the Journal of Oral Pathology & Medicine. "The ontology of these 47 genes was classified into 10 categories. To validate the microarray data, the expression of genes, including TGFBI, FADD and DUSP1 was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR)." "By hierarchical clustering analysis, the nine cases were divided into two clusters," published data indicated. "The 47 genes are suggested as having a functional significance in oral squamous cell carcinogenesis." "It is also suggested that the gene expression patterns by hierarchical clustering analysis can represent degrees of differentiation," according to the report. "The postoperative recovery was uneventful and patients were free from tumor after surgery." "In the future, on the occasion when the time comes that the number of cases accumulated for microarray increases and each case is observed more over a long term, these data of 5-year survival rate will be added. Thereby, it will become possible to represent the malignancy of OSCC by these gene expression patterns," the researchers concluded. Tomioka and coauthors published their study in the Journal of Oral Pathology & Medicine (Gene expression analysis by cDNA microarray in oral squamous cell carcinoma. J Oral Pathol Med, 2006;35(4):206-211). For additional information, contact H. Tomioka, Tokyo Medical & Dental University, Dept. of Oral Restitution, Division of Oral Health Sciences, Graduate School, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138549, Japan. The publisher of the Journal of Oral Pathology & Medicine can be contacted at: Blackwell Publishing, 9600 Garsington Rd., Oxford OX4 2DQ, Oxon, England. Keywords: Tokyo, Japan, Carcinogenesis, DNA Microarray, DNA Research, Diagnostics, Gene Expression, Genetics, Genomics, Oncology, Oral Cancer, Oral Squamous Cell Carcinoma, Proteins, Proteomics. This article was prepared by Science Letter editors from staff and other reports. Copyright 2006, Science Letter via NewsRx.com. |